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KMID : 0368419890320040275
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Journal of Plant Biology
1989 Volume.32 No. 4 p.275 ~ p.283
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Purification and Properties of β-1, 4-endoglucanase from Tobacco Suspension Cultured Cells
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Abstract
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β-1,4-endoglucanase(EC 3,2,1,4) was isolated and purified from Nicotiana tabaccum L. Var. Virginia 115 suspension cultured cells. The molecular weight as estimated by Sephadex G-100 was about 14,000. The optimum pH for activity was 5.4. The Km value for carboxymethyl cellulose was 0.18 ㎎/unit and the enzyme was quite resistant to heating. Polyamine did not affect the activity of β-1,4-endoglucanase in vitro but the activity increased drastically when polyamines were added in the suspension culture medium. It is suggested that increase in β-1,4-endoglucenase activity due to polyamine might be related to growth of the plant.
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